A new fluorescence corneal sectioned microscopy technique for in situ detection of Acanthomoeba published in Biomedical Optics Express
Acanthomeaba keratitis is a morbid corneal infection with challenging diagnosis. We present a new technique which combines the development of a new fluorescent marker, selective for Acanthomoeba with fluorescent sectioned (structured illumination) microscopy which allows efficient in situ detection of acanthomoebas implanted in the corneal stroma of a rabbit model cornea. The study is the result of a collaboration between the Visual Optics and Biophotonics Lab, with physico-chemist from the IQRS and IQO-CSIC, biologists from CIB-CSIC and clinicians from IOBA/FIO.
Full reference: Marcos et al. Fluorescent labeling of Acanthamoeba assessed in situ from corneal sectioned microscopy, Biomedical Optics Express, Vol. 3, Issue 10, pp. 2489-2499 (2012)