Rose Bengal Corneal Cross-linking : Clinical and Histological changes published in IOVS
Following studies on the corneal biomechanical changes induced by Rose Bengal CXL in vivo in an experimental model on rabbits, we report on the histology and clinical observations in the cornea in a 60-day follow up, in comparison with contralateral eyes left after control (de-epithealized). It was found that cell death was restricted to the anterior central stroma, and the cellular decrease did not differ significantly between RGX and DE corneas. Cell proliferation in the epithelium and stroma appeared at 2 days. In both DE and RGX corneas, recovery of the epithelium was complete at day 30, although cell repopulation of the stroma was not complete at 60 days. Rose Bengal CXL appears therefore an efficient and safe method to increase corneal stiffness, with significant advantages over traditional UV Cross-linking (use of visible light, less penetration and much faster application). The work is the result of a collaboration between Wellman Center of Photomedicine, MGH-Harvard Medical School (Irene Kochevar’s Lab), Universidad de Valladolid (Carmen Martinez’s Lab) and Instituto de Optica CSIC (VioBio Lab).
Full reference: Patricia Gallego-Muñoz; Lucía Ibares-Frías; Elvira Lorenzo; Susana Marcos; Pablo Peréz-Merino; Nandor Bekesi; Irene E. Kochevar; M. Carmen Martínez-García. Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and Histologic Study. Investigative Ophthalmology & Visual Science July 2017, Vol.58, 3471-3480.