Quantization of collagen organization in the stroma with a new order coefficient, published in Biomedical Optics Express

HorizontalFigure6

A new order coefficient was introduced to quantize the differences in organization between three different types of treatment applied to porcine corneas: untreated corneas, UVX crosslinked corneas, and corneas treated with riboflavin.  Collagen fibers in the stroma were imaged through a depth of 700 mm using forward-scattering second harmonic generation microscopy.  Analysis points based on the orientation of the fibers were calculated and the number of points falling within 10° analysis windows was counted.  From these points, the order coefficient was calculated as the normalized variance of the number of analysis points in each window.  This quantization showed that the UVX crosslinked corneas were more ordered up to a depth of 400 mm in the stroma.

Full reference: James A. Germann, Eduardo Martínez-Enriquez and Susana Marcos. “Quantization of collagen organization in the stroma with a new order coefficient” Biomed. Opt. Express 9, 173-189 (2018).

Full article here.

Advertisements

Posted on December 13, 2017, in General. Bookmark the permalink. Leave a comment.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

%d bloggers like this: